| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Lyla E (17)
|
MDCAC |
56.58 |
55.12 |
-1.46 |
| 2 |
Greenwood, Kaiylin R (18)
|
MDYCM |
57.34 |
57.05 |
-0.29 |
| 3 |
Gurri, Maddalena G (15)
|
MDMBK |
57.40 |
57.57 |
0.17 |
| 4 |
Randolph, Audrey A (15)
|
MDCAC |
59.62 |
57.71 |
-1.91 |
| 5 |
Quaresma, Eliana M (15)
|
MDCAC |
58.69 |
58.37 |
-0.32 |
| 6 |
Foveaux, Caileigh N (16)
|
MDCAC |
1:02.25 |
59.56 |
-2.69 |
| 7 |
Barbe, Messina E (15)
|
MDYCM |
59.67 |
59.64 |
-0.03 |
| 8 |
Linger, Gabriela M (15)
|
MDROCK |
1:01.51 |
1:01.81 |
0.30 |
| 9 |
Longmore, Isabella R (17)
|
MDHAWK |
1:00.36 |
1:02.28 |
1.92 |
| 10 |
David, Zoe A (15)
|
MDOPST |
1:01.77 |
1:02.61 |
0.84 |
| 11 |
Chen, Sophia (16)
|
MDCAC |
1:05.48 |
1:05.44 |
-0.04 |
| 12 |
Mogavero, Anna T (17)
|
MDCAC |
1:06.01 |
1:05.45 |
-0.56 |
| 13 |
Funk, Zoe G (16)
|
MDYCM |
1:04.08 |
1:07.18 |
3.10 |
| 14 |
Longmore, Gabrielle L (15)
|
MDHAWK |
1:06.82 |
1:10.84 |
4.02 |